全文获取类型
收费全文 | 420篇 |
免费 | 19篇 |
国内免费 | 21篇 |
专业分类
系统科学 | 1篇 |
丛书文集 | 14篇 |
教育与普及 | 2篇 |
现状及发展 | 25篇 |
综合类 | 418篇 |
出版年
2022年 | 1篇 |
2021年 | 10篇 |
2020年 | 3篇 |
2019年 | 7篇 |
2018年 | 6篇 |
2017年 | 8篇 |
2016年 | 7篇 |
2015年 | 18篇 |
2014年 | 19篇 |
2013年 | 16篇 |
2012年 | 18篇 |
2011年 | 18篇 |
2010年 | 19篇 |
2009年 | 22篇 |
2008年 | 16篇 |
2007年 | 25篇 |
2006年 | 19篇 |
2005年 | 20篇 |
2004年 | 14篇 |
2003年 | 16篇 |
2002年 | 19篇 |
2001年 | 14篇 |
2000年 | 26篇 |
1999年 | 14篇 |
1998年 | 16篇 |
1997年 | 7篇 |
1996年 | 9篇 |
1995年 | 14篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 9篇 |
1991年 | 10篇 |
1990年 | 5篇 |
1989年 | 7篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1980年 | 1篇 |
排序方式: 共有460条查询结果,搜索用时 312 毫秒
1.
Aldose reductase and aldehyde reductase belong to the aldo-keto reductase superfamily of enzymes whose members are responsible
for a wide variety of biological functions. Aldose reductase has been identified as the first enzyme involved in the polyol
pathway of glucose metabolism which converts glucose into sorbitol. Glucose over-utilization through the polyol pathway has
been linked to tissue-based pathologies associated with diabetes complications, which make the development of a potent aldose
reductase inhibitor an obvious and attractive strategy to prevent or delay the onset and progression of the complications.
Structural studies of aldose reductase and the homologous aldehyde reductase in complex with inhibitor were carried out to
explain the difference in the potency of enzyme inhibition. The aim of this review is to provide a comprehensive summary of
previous studies to aid the development of aldose reductase inhibitors that may have less toxicity problems than the currently
available ones.
Received 4 December 2006; received after revision 12 February 2007; accepted 20 April 2007 相似文献
2.
Pyruvate dehydrogenase kinase regulatory mechanisms and inhibition in treating diabetes, heart ischemia, and cancer 总被引:2,自引:2,他引:0
The fraction of pyruvate dehydrogenase complex (PDC) in the active form is reduced by the activities of dedicated PD kinase
isozymes (PDK1, PDK2, PDK3 and PDK4). Via binding to the inner lipoyl domain (L2) of the dihydrolipoyl acetyltransferase (E2
60mer), PDK rapidly access their E2-bound PD substrate. The E2-enhanced activity of the widely distributed PDK2 is limited
by dissociation of ADP from its C-terminal catalytic domain, and this is further slowed by pyruvate binding to the N-terminal
regulatory (R) domain. Via the reverse of the PDC reaction, NADH and acetyl-CoA reductively acetylate lipoyl group of L2,
which binds to the R domain and stimulates PDK2 activity by speeding up ADP dissociation. Activation of PDC by synthetic PDK
inhibitors binding at the pyruvate or lipoyl binding sites decreased damage during heart ischemia and lowered blood glucose
in insulin-resistant animals. PDC activation also triggers apoptosis in cancer cells that selectively convert glucose to lactate.
Received 25 August 2006; received after revision 20 November 2006; accepted 20 December 2006 相似文献
3.
脑梗塞急性期胰岛素与血糖水平的研究 总被引:1,自引:0,他引:1
选择158例脑梗塞患者为观察组,另选择100例非脑梗塞患者为对照组。24h内抽取两组患者的静脉血测定空腹胰岛素值、空腹血糖值,并计算出胰岛素敏感性指数。对两组的胰岛素值、血糖及胰岛素敏感指数进行统计学比较。结果表明,观察组胰岛素水平、血糖水平明显高于对照组(P<0.01),胰岛素敏感性指数明显低于对照组(P<0.01),脑梗塞急性期胰岛素及血糖水平增高,胰岛素敏感性指数降低。 相似文献
4.
J. -M. Weber 《Cellular and molecular life sciences : CMLS》1992,48(6):557-564
The study of metabolic fuel provision and its regulation has reached an exciting stage where specific molecular events can be correlated with parameters of the organism's ecology. This paper examines substrate supply pathways from storage sites to locomotory muscle mitochondria and discusses ecological implications of the limits for maximal flux through these pathways. The relative importance of the different oxidative fuels is shown to depend on aerobic capacity. Very aerobic, endurance-adapted animals such as long distance migrants favor the use of lipids and intramuscular fuels over carbohydrates and circulatory fuels. The hypothesis of functional co-adaptation between oxygen and metabolic fuel supply systems allows us to predict that the capacity of several biochemical processes should be scaled with maximal oxygen consumption. Key enzymes, transmembrane transporter proteins, glucose precursor supply and soluble fatty acid transport proteins must all be geared to support higher maximal glucose and fatty acid fluxes in aerobic than in sedentary species. 相似文献
5.
电聚合吡咯膜修饰的葡萄糖氧化酶电极 总被引:1,自引:1,他引:0
自制的铂丝电极经铂化处理后,将葡萄糖氧化酶与单体吡咯用循环伏安法共聚合到电极上,铂化后电极表面积的增大增加了固定的酶量,同时也增大了电极对H2O2的催化能力,使电极对葡萄糖的响应电流增大到7.5mA/(mol·L^-1),还测试了聚合液中酶浓度、pH值和聚合时间等因素对电极特性的影响。 相似文献
6.
再生丝心蛋白和羧甲基纤维素复合膜的特性及其在有机相葡萄糖生物传感器中的应用 总被引:1,自引:0,他引:1
用再生丝心蛋白和羧甲基纤维素构成的复合膜作为固定葡萄糖氧化酶的基质,并用红外光谱测试了该复合膜的结构,用扫描电子显微镜观察了其形态.用上述复合膜材料固定葡萄糖氧化酶和羧酸二茂铁(或7,7,8,8-四氰代二甲基苯醌,TCNQ)制成的葡萄糖生物传感器具有较好的灵敏度与稳定性 相似文献
7.
葡萄糖氧化酶及过氧化氢酶的生物合成 总被引:7,自引:3,他引:4
刘建忠 《中山大学学报(自然科学版)》1998,37(1):68-72
研究了黑曲霉细胞的生长及其葡萄糖氧化酶、过氧化氢酶的生物合成.CaCO3是2种酶的产酶诱导因子,其产酶模式都是延续合成型,最大比生长速度μmax、菌体得率Y(X/S)、葡萄糖氧化酶及过氧化氢酶合成得率Y(GOD/S)、Y(CAT/S)分别为0.097h-1、0.075g.g-1、29.34μmol·min-1·g-1、389.17μmol·min-1·g-1.也研究了各种物质对葡萄糖氧化酶及过氧化氢酶活力的影响,金属离子对葡萄糖氧化酶有抑制,阴离子对葡萄糖氧化酶和过氧化氢酶有抑制,还原性小分子化合物是葡萄糖氧化酶的激活剂 相似文献
8.
Ferrocene-Nafion修饰厚膜碳糊电极的葡萄糖传感器研究 总被引:1,自引:0,他引:1
以基于丝网印刷技术制作的厚膜碳糊电极为基底电机,用二茂铁为介体、Nafion修饰厚膜碳糊电极制备了葡萄糖传感器.Nafion涂于电极表面上形成的膜具有强的附着力,防止了二茂铁及酶的流失,电极稳定性提高.并且由于低的工作电位(+025vsSCE)和Nafion膜的阳离子交换作用,基本上消除了电活性物质(抗坏血酸、尿酸)的干扰,具有防污能力.该酶传感器的检测上限可达18mmol/L,响应时间小于60s. 相似文献
9.
The cyclicity and intensity of gastric motlity were examined following glucose injection into the portal vein with an intragastric balloon in anesthetized rats. Enhanced gastric motility caused by insulin administration was influenced by 4 mM glucose (25 l) injected into the portal vein; glucose provoked a shift in the cyclicity power spectrum without any change in intensity. The peak power spectrum shifted from 4.0–5.0 cpm to 2.0–3.0 cpm. Hepatic branch vagotomy abolished the response.The results suggest that glucose signals in the hepatic vagal branch modulate the cyclicity of gastric motiligy. 相似文献
10.
葡萄糖对油制气废水生物降解影响的初步研究 总被引:1,自引:0,他引:1
采用3种驯化分离的菌种S-2、Y-3、XH-3及其混合菌M-3,以葡萄糖作为共代谢基质,研究了葡萄糖对各菌降解油制气废水的影响.结果表明:加入葡萄糖,可使COD、氨氮、可萃取有机物的去除率分别提高16.9%~26.5%、17.5%~31.2%、15.2%~23.3%;但在所采用的时间范围内,酚类化合物的去除率降低.加入葡萄糖,各菌对废水中芳烃类化合物的去除率提高17.7%~21.7%.除了维持无营养物条件下对芳环数≤3芳烃类化合物的良好降解能力外,对芳环数为4~6芳烃类化合物的降解能力也有所提高. 相似文献